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Antiviral activity of Aplysia dactylomela ink

Figueiredo CG1, Figueiredo CA2, de Oliveira MI2, Silva Jr ER2, Mendonça RZ1, Toledo-Piza ART1

1 Laboratório de Parasitologia, Instituto Butantan, Brasil; 2 Núcleo de Doenças Respiratórias, Instituto Adolfo Lutz , Brasil

Introduction:  Mollusks are found in the sea, in fresh water and on land rely only on his innate immunity to fend off pathogens. Much of the molecules are in preclinical or clinical studies were isolated from sessile animals or reduced capacity of movement, soft body and devoid of physical defense structures, and non-calcareous sponges, sea squirts, soft corals and mollusks shucked. This thanks to the ecological function of these molecules to ensure the success of the host in the competition for space or defense against predators or pathogenic micro-organisms.The Species of the genus Aplysia, known as hares or sea- slugs secrete a purple ink, from the red algae pigments which feed rich in chemical compounds responsible for its defense. From that material they were isolated several proteins with antibacterial activity, cytolytic, antitumor and hemagglutination. Measles is a single-stranded RNA and enveloped paramyxovirus that causes an acute generalized infection. Rubella is a disease caused by a togavirus, single-stranded RNA enveloped viruses and transmitted by the respiratory route. Cause mild fever and small red spots on the skin. Recent outbreaks have occurred in vaccinated populations in developing countries. Objectives: This work proposes to demonstrate the antiviral activity of Aplysia dactylomela ink. Methods: Ink samples were collected from 10 mollusks from Caucaia (CE-Brazil). After lyophilization, sample was solubilized in ultra-pure water supplemented with trifluoroacetic acid (0.1%), pre-purified in a STRATA C18 column and submitted to experiments after lyophilization. In order to evaluate the viral growth inhibition, bioassays were carried out on plaque and analysis by quantitative PCR using SIRC and Vero cells infected with viruses of measles and rubella, respectively. Results and Discussion: Crude ink inhibited viral growth when the cultures with measles and rubella were treated with a concentration of 13μg/μl and 8.6μg/μl, respectively. The results demonstrate that A. dactylomela ink was able to inhibit the growth of both viral types. In the future, purification process will be carried out to isolate and identify the molecules responsible for this activity.

Supported by CNPq



10 - BIODIVERSITY 21th Annual Scientific Meeting of Instituto Butantan.